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Extra resources for Amino Acids in Animal Nutrition
Schneider (1989) of the company Beckman gave an overview of separating conditions and reproducibility of the technique, Drnevich and Vary (1993) used it to analyse blood plasma samples, Ikeda et al. (1993) employed this reagent for the determination of hydroxyproline and proline in collagen and Krause et al. (1995) tested amino acids and biogenic amines in biological samples and food. Also worth mentioning is the fluorescence-active, long-established reagent dansyl chloride which was used by Sanz et al.
R. (1999) HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. Journal of Agricultural and Food Chemistry 47, 5100–5107. D. R. (2001) Free amino acids in dark- and white-muscle fish as determinated by o-phthaldialdehyde precolumn derivatization. Journal of Food Science 66, 72–77. 12 Amino Acids in Feeds. In: Official Methods of Analysis, 16th edn. AOAC International, Arlington, Virginia, chapter 4, 4–4H. -C. and Toldra, F. (1991) Deproteinization for HPLC amino acid analysis in fresh pork muscle and dry-cured ham.
Grunau and Swiader (1992) and Moeller (1993) used IEC columns in HPLC machines with two- or three-buffer gradient elution; Le Boucher et al. (1997) subjected the Hitachi L-8500A, a new amino acid analyser, to intensive tests with biological fluids for performance in peak reproducibility, retention time stability and linear concentration, with good results. 23 Other separation and detection techniques of underivatized amino acids The separation of amino acids by means of anion exchange chromatography was also tested, often combined with pulsed amperometric detection (PAD).